Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
نویسندگان
چکیده
منابع مشابه
Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
متن کاملNovel multiplex real-time PCR diagnostic assay for identification and differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis complex strains.
Tuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely initiation of antibiotic treatment, while differentiation between members of the complex may be important to guide the appropriate antibiotic treatment and provide epidemiological information. In this study, a multiplex real-time PCR diagnostic...
متن کاملA novel multiplex real - time PCR diagnostics assay for the identification and 1 differentiation of Mycobacterium tuberculosis , Mycobacterium canettii and 2 the Mycobacterium tuberculosis complex 3 4
26 Tuberculosis (TB) in humans is caused by members of the Mycobacterium 27 tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely 28 initiation of antibiotic treatment, while differentiation between members of the 29 complex may be important to guide the appropriate antibiotic treatment and provide 30 epidemiological information. 31 In this study, a multiplex real-t...
متن کاملIdentification of Mycobacterium bovis Isolates by a multiplex PCR
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
متن کاملZoonotic tuberculosis (Mycobacterium bovis):
In view of the considerable and continuing public health significance of Mycobacterium bovis infection in humans and animals, WHO convened a meeting on zoonotic tuberculosis in Geneva in November 1993. The participants at the meeting reviewed the human and animal tuberculosis situation worldwide, discussed the zoonotic aspects of M. bovis infection in United Republic of Tanzania and Zambia, exc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2014
ISSN: 1517-8382
DOI: 10.1590/s1517-83822014000300012